Uncompetitive Inhibition

When inhibitors bind allosterically to the ES complex to form an ESI complex

• $K_m$ decreased
  • It’s easier to reach half $v_{\text{max}}$ because the inhibitor causes ES to stay stuck together
• $v_{\text{max}}$ decreased
  • Inhibitor is converting all ES to inactivated form, so there’s a cap added to $v_{\text{max}}$

Lineweaver-Burk Plot

Mnemonic

• The U looks like two arrows pointing downward, indicating decreasing $K_m$ and $v_{\text{max}}$
• The U is horizontally symmetric, so this corresponds to the inhibitor only binding the ES complex based on the reaction diagram looking like a symmetric T