Affinity Chromatography
#Biochemistry
Specially made antibodies for the target protein are inserted in the column to grab them. They are then later eluted out
Types
| Ligand | Target |
|---|---|
| $\displaystyle \ce{Ni^{2+}}$ | His6-tagged POI |
| Avidin | Biotin |
| Glutathione | GST-POI |
| polyT | polyA-mRNA |
Parts
- Feed
- Initial sample
- Flow-Through
- Part of sample that flows through column
- Wash
- Buffer that goes through the column to wash out any remaining non-specific protein
- Eluate (Elution)
- Final purified fraction with protein of interest