Electrophoresis

#Biochemistry

Types

• Native PAGE
• SDS PAGE
• Isoelectric Focusing

$\displaystyle v=\frac{Ez}{f}$

• $\displaystyle v$ is the migrational velocity of the protein in the medium
• $\displaystyle E$ is the electric field strength
• $\displaystyle z$ is the net charge on the protein
• $\displaystyle f$ is the frictional coefficient on the protein, which is typically dependent on the mass and shape of the molecule
• Can be derived by $\displaystyle \sum F=zE-fv=ma=0$ due to $\displaystyle a\rightarrow0$ at terminal velocity (check this later...)