Protein Overexpression

#Biochemistry

Chemicals Required

• Inducer
  • Deactivates the repressor on the operon and allows protein to be made
  • Examples include IPTG
• LB Broth
  • Provides nutrients to the bacteria
    • Tryptone (peptides)
    • Yeast extract
    • NaCl
    • Vitamins
    • Metal ions
    • Screening agent (e.g. ampicilllin)