Uncompetitive Inhibition

#Biochemistry

When inhibitors bind allosterically to the ES complex to form an ESI complex

• $\displaystyle K_{m}$ decreased
  • It's easier to reach half $\displaystyle v_{\text{max}}$ because the inhibitor causes ES to stay stuck together
• $\displaystyle v_{\text{max}}$ decreased
  • Inhibitor is converting all ES to inactivated form, so there's a cap added to $\displaystyle v_{\text{max}}$

Lineweaver-Burk Plot

Mnemonic

• The U looks like two arrows pointing downward, indicating decreasing $\displaystyle K_{m}$ and $\displaystyle v_{\text{max}}$
• The U is horizontally symmetric, so this corresponds to the inhibitor only binding the ES complex based on the reaction diagram looking like a symmetric T